Journal: eLife

Article Title: Chronic stress impairs autoinhibition in neurons of the locus coeruleus to increase asparagine endopeptidase activity

doi: 10.7554/eLife.106362

Figure Lengend Snippet: ( A, B ) Confocal images of LC neurons showing immunoreactivities for TH and α2A-ARs, together with a merged one in non-stress and 3-day RS mice. Arrowheads indicate the membrane regions of TH-positive neurons, along which α2A-ARs were differentially expressed between the control and the RS mice. Asterisks indicate LC neurons. ( C ) Western blotting analyses showing the expression of α2A-ARs in non-stress and 3-day RS mice ( n =6 and 7, respectively). Unpaired t-test, † p<0.001. ( D, E ) Confocal images of LC neurons in non-stress and RS mice. Upper panels from left to right showing the respective immunoreactivity for TH, α2A-ARs (with rabbit anti-α2A-AR) and Na + -K + -pump, and an enlarged image of the region enclosed with a rectangle (arrowheads) in its immediate left panel. Lower panels from left to right showing the merged image of TH and α2A-ARs, that of TH, α2A-ARs, and Na + -K + -pump, that of α2A-ARs and Na + -K + -pump, and an enlarged image of the region enclosed with a rectangle in its immediate left panel. ( F, G ) Western blotting analyses showing expressions of α2A-ARs and Na + -K + -pump in membrane fraction and those of α2A-ARs and β-actin in cytosol fraction in non-stress and 5-day RS mice ( n =3 and 3 samples, respectively). Each sample represents the analysis result in the LC tissues obtained from two to three mice. Membrane; unpaired t-test, † p=0.016. Cytosol; unpaired t-test, † p=0.083. Figure 3—source data 1. Original files for the western blot analysis in . Figure 3—source data 2. PDF containing the original blots in with the relevant bands clearly labeled. Figure 3—source data 3. Data used for graphs presented in .

Article Snippet: Antibody , Rabbit anti-α2A AR (polyclonal) , alomone , Cat# AAR-020, RRID: AB_10687546 , WB (1:500).

Techniques: Confocal, Membrane, Control, Western Blot, Expressing, Analysis, Labeling, Immunofluorescence

Journal: eLife

Article Title: Chronic stress impairs autoinhibition in neurons of the locus coeruleus to increase asparagine endopeptidase activity

doi: 10.7554/eLife.106362

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit anti-α2A AR (polyclonal) , alomone , Cat# AAR-020, RRID: AB_10687546 , WB (1:500).

Techniques: